Journal of PhytoPharmaceutical and Advanced Chemistry
Short Name: JPPAC• ISSN: 3139-9266• Peer-Reviewed Open Access
Evaluation of Antibacterial Activity of Essential Oil Extracted from the Peels of Citrus limetta Against the Enteric Fever Pathogen Salmonella enterica serovar Typhi
Abstract
Horticultural waste, though historically overlooked, serves as a valuable reservoir of bioactive compounds. Addressing the valorization of citrus byproducts, this study investigates the extraction and antimicrobial properties of essential oils derived from sweet lime (Citrus limetta) peels. Essential oils were extracted via hydrodistillation, isolated, and initially characterized using thin-layer chromatography (TLC). Subsequent qualitative profiling was conducted using Gas Chromatography-Mass Spectrometry (GC-MS), which revealed a diverse array of bioactive compounds, with limonene identified as a predominant constituent. The antimicrobial efficacy of the extracted oil was evaluated against clinical isolates of Salmonella typhi, the causative agent of enteric fever, utilizing the agar well diffusion method to assess zones of inhibition. Given the significant antimicrobial properties of limonene, the extract demonstrated strong inhibitory effects. To our knowledge, this is the first study to establish the in vitro antibacterial activity of sweet lime essential oil against Salmonella typhi, highlighting its potential therapeutic application as a natural treatment alternative for typhoid fever.
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Journal of PhytoPharmaceutical and Advanced Chemistry
An international, peer-reviewed journal integrating phytopharmaceutical sciences, natural products, and advanced pharmaceutical chemistry.
This peer-reviewed publication is indexed directly from the publisher repository adhering to Open Archives Initiative (OAI-PMH) metadata standards, Crossref DOI schema, and Google Scholar Highwire Press protocols.