Combined Effect of Leaf Extracts of Mangifera indica and Plumeria alba on Anti-Arthritic Activity

This study was undertaken to investigate any combined antiarthritic effect between the methanolic extracts of leaves of Mangifera indica L. and Plumeria alba L. Both the extracts and their mixture (in the ratio 1:1) were studied for their total flavonoid content (TFC), total phenolic content (TPC) and antioxidant activity. In-vitro antiarthritic activity was also carried out using protein denaturation method. Both the individual extracts showed significant antioxidant activity but the mixture showed more activity compared to the individual extracts. The significant amount of phenol content in the extracts might be responsible for their antioxidant property. The results showed that the mixed extract exhibited the highest TPC (99.06 mg/g), surpassing Mangifera indica L. (81.40 mg/g) and Plumeria alba L. extract (75.25 mg/g). Similarly, the results of total flavonoid content revealed that the mixed extract exhibited the highest TFC (123.42 mg/g), as compared to Mangifera indica L. (98.42 mg/g) and Plumeria alba L. extract (78.22 mg/g). Both the individual extracts and their mixture showed a dose dependent inhibition of the protein denaturation. The values of IC50 for Mangifera indica L. extract and Plumeria alba L. extract were found to be 357.10 and 416.48 μg/mL, respectively. IC50 value of mixture was 246.36 μg/mL which was close to the IC50 value of standard drug diclofenac sodium (236.04 μg/mL). It can be concluded that a combination of Mangifera indica L. and Plumeria alba L. might exert an anti-arthritic effect due to a possibility of multifaceted biological activities shown by chemically different phytochemicals.

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